Tumour-associated high endothelial venules drive portal-specific immune evasion in lymph nodes via ALOX12 [Metastatic lymph node]

High-endothelial-venules (HEVs) offer another entry point for tumor metastasis in mice. To investigate whether HEVs and non-specialized blood vessels exert different effects on disseminating tumor cells, we enriched EO771 derivatives HEVM3 (preferentially metastasize through HEVs) cells and BVM3 (preferentially metastasize through blood vessels) cells. To further explore potential factor(s) that influence tumor cells, we isolated blood vessel endothelial cells (BECs) in metastatic lymph nodes from six C57BL/6J mice and analyzed them with single-cell RNA sequencing. Overall design: BECs in metastatic lymph nodes from six C57BL/6J mice were analyzed with single-cell RNA sequencing.