DSB inducing agents induce double strand DNA breaks

Reactive oxygen species (ROS) induce DNA double-strand breaks (DSBs) (Yu and Anderson 1997) in cells undergoing oxidative stress. In addition to ROS, DSBs can also be directly generated by ionizing radiation. Agents that interfere with the progression of replication forks, such as topoisomerase poisons used in chemotherapy, induce DSBs indirectly (Curtin 2012).

活性氧物种（ROS）可在经历氧化应激的细胞中诱导DNA双链断裂（DSBs）（Yu和Anderson，1997年）。除ROS外，电离辐射亦能直接引发DSBs。干扰复制叉进展的试剂，如化疗中使用的拓扑异构酶抑制剂，可间接诱导DSBs（Curtin，2012年）。