High-throughput sequencing (HTS) techniques are of great value for the investigation of microbial communities, and have been extensively used to study the gut microbiome. While most studies focus on t
Hepes-glutamic acid buffer-mediated organic solvent protection effect (HOPE)-fixation has been introduced as an alternative to formalin fixation of clinical samples. Beyond preservation of morphologic
Additional file 8: Table S6. Genes differentially expressed between cryopreserved and freshly profiled warm-dissociated kidney single-cell suspensions (Seurat Wilcoxon test [30] with thresholds of log