Pisum sativum cultivar:Aragorn Raw sequence reads

Recent advances in genome sequencing, genotyping and computational strategies have made it possible and affordable to develop expressed sequence tag (EST) libraries that contain hundreds of thousands to millions of sequences. ESTs, sequences of cDNA made by reverse transcription of mRNAs, are particularly valuable for discovering new genes, for obtaining data on gene expression and regulation, for constructing genomic maps and for the development of single nucleotide polymorphism (SNP) markers. The goal of this project is to develop a reference transcriptome of ESTs from multiple tissues/organelles and growth stages of Pisum sativum cv ‘Aragorn’ and develop an EST database.

近年来，基因组测序、基因分型和计算策略的显著进展使得构建包含数十万至数百万序列的表达序列标签（EST）库成为可能且经济实惠。ESTs，即通过反转录mRNA得到的cDNA序列，对于发现新基因、获取基因表达和调控数据、构建基因组图谱以及开发单核苷酸多态性（SNP）标记具有极高的价值。本项目的目标是开发出来自豌豆品种‘Aragorn’多个组织和细胞器以及不同生长阶段的EST参考转录组，并构建EST数据库。