Supplemental Material for Sorgini et al., 2021

Supplemental Tables S1-S5.Table S1: Recombinant Inbred Lines used for single family QTL mapping of leaf δ13C, SLA, and ionomics.Table S2: Recombinant Inbred Lines used for leaf δ13C joint linkage mapping.Table S3: Lines used for leaf δ13C GWAS.Table S4: Significant QTL identified in ionomics analyses.Table S5: Weather data for field sites.
Supplemental Figure 1 NAM RIL Transgressive Segregation. NAM RIL families CML103 (A),
CML333 (B), NC358 (C), and Tx303 (D) were sorted by δ13C and plotted. Parental lines are
shown in red.

Supplemental Figure 2 Element and δ13C Full Correlation
Matrix. A full correlation matrix of element and δ13C
mean values is show. The diagonal displays histograms of each dataset.
Pearson’s r is shown in the upper panel. Scatter plots and best fit line are
shown in the lower panel.

Supplemental Figure 3 Element QTL Mapping by Chromosome. Significant element QTL are shown by maize chromosomes 1 through
10 on the x-axis (in cM). Each NAM RIL family is represented by a symbol;
CML103 (○), CML333 (x), NC358 (□), and Tx303 (Δ). Each element is designated by
color. Significance thresholds (dashed
horizontal line) were determined using 200 permutations, alpha=0.05 for each
QTL independently.

Supplemental Figure 4
Genome Wide Association Study for δ13C. Manhattan plot showing significance of SNPs
derived from a mixed linear model using the Bayesian information criterion to
select the optimal number of principal components. The significance threshold
represents the Bonferroni correction of familywise error rate.