Exploring the Impact of SOD1 Transcript Variants on Amyotrophic Lateral Sclerosis

The database includes the raw data of the article "Exploring the Impact of SOD1 Transcript Variants on Amyotrophic Lateral Sclerosis". Aim of this work was to investigate the role of SOD1 transcripts in amyotrophic lateral sclerosis. The database contains the data obtained by the following evaluations:
 a) immunofluorescence analysis for the evaluation of total, transfected and endogenous SOD1 in transfected SHYSY5Y and Hela cells, b) Multiplex analysis for the evaluation of SOD1 transcripts in SYSY5Y cells and in PBMCs patients, c) correlation analysis for the correlation between SOD1 transcripts and ages of patients, ALSFRS_R value, PRB value and PRL value.
We found that the shortest transcript is upregulated in stress condition in both the cellular model and the patients' cells. Moreover, we found a possible correlation between the SOD1 short transcript and the severity of the pathology, which correlates also with the age of patients at symptoms onset. No correlation was found between SOD1 transcripts and the progression of the disease. Our data suggest a toxic effect of SOD1 short transcript in patients affects by amyotrophic lateral sclerosis.