Table_4_Comparative Analysis of the Gut Microbiota of Adult Mosquitoes From Eight Locations in Hainan, China.xlsx

The midgut microbial community composition, structure, and function of field-collected mosquitoes may provide a way to exploit microbial function for mosquito-borne disease control. However, it is unclear how adult mosquitoes acquire their microbiome, how the microbiome affects life history traits and how the microbiome influences community structure. We analyzed the composition of 501 midgut bacterial communities from field-collected adult female mosquitoes, including Aedes albopictus, Aedes galloisi, Culex pallidothorax, Culex pipiens, Culex gelidus, and Armigeres subalbatus, across eight habitats using the HiSeq 4000 system and the V3−V4 hyper-variable region of 16S rRNA gene. After quality filtering and rarefaction, a total of 1421 operational taxonomic units, belonging to 29 phyla, 44 families, and 43 genera were identified. Proteobacteria (75.67%) were the most common phylum, followed by Firmicutes (10.38%), Bacteroidetes (6.87%), Thermi (4.60%), and Actinobacteria (1.58%). The genera Rickettsiaceae (33.00%), Enterobacteriaceae (20.27%), Enterococcaceae (7.49%), Aeromonadaceae (7.00%), Thermaceae (4.52%), and Moraxellaceae (4.31%) were dominant in the samples analyzed and accounted for 76.59% of the total genera. We characterized the midgut bacterial communities of six mosquito species in Hainan province, China. The gut bacterial communities were different in composition and abundance, among locations, for all mosquito species. There were significant differences in the gut microbial composition between some species and substantial variation in the gut microbiota between individuals of the same mosquito species. There was a marked variation in different mosquito gut microbiota within the same location. These results might be useful in the identification of microbial communities that could be exploited for disease control.

对野外采集的蚊虫中肠微生物群落组成、结构及功能的分析，可能为利用微生物功能以控制蚊媒疾病提供一条途径。然而，关于成年蚊虫如何获取其微生物组、微生物组如何影响生命周期性状以及微生物组如何影响群落结构，目前尚不明确。本研究采用 HiSeq 4000 系统及 16S rRNA 基因 V3−V4 高变区，对来自八个栖息地、包括白纹伊蚊、埃及伊蚊、淡色库蚊、致倦库蚊、寒冷库蚊和黄足伊蚊等501个野外采集的成年雌蚊中肠细菌群落进行了分析。经过质量过滤和物种丰富度稀释，共识别出1421个操作分类单元，隶属于29个门、44个科和43个属。变形菌门（75.67%）是最常见的门类，其次是厚壁菌门（10.38%）、拟杆菌门（6.87%）、热微菌门（4.60%）和放线菌门（1.58%）。在所分析的样本中，立克次体科（33.00%）、肠杆菌科（20.27%）、乳杆菌科（7.49%）、气单胞菌科（7.00%）、热杆菌科（4.52%）和莫拉氏菌科（4.31%）的属在数量上占主导地位，占总属数的76.59%。本研究对海南岛六个蚊种的中肠细菌群落进行了特征化分析。不同地点的蚊种肠道细菌群落组成和丰度存在差异。一些蚊种间的肠道微生物组成存在显著差异，同一蚊种个体间的肠道微生物群也有显著变异。在同一地点，不同蚊种肠道微生物群也存在明显差异。这些研究结果可能有助于识别可用于疾病控制的微生物群落。