Hydrogen peroxide tolerance assay of Streptococcus gordonii
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Overnight cultures of <i>Streptococcus gordonii </i>were harvested and resuspended in RPMI medium (pH 7.0), and then 5x10<sup>5</sup> cells/ml of bacteria were transferred to RPMI medium (pH 5.0) supplemented with H<sub>2</sub>O<sub>2</sub> (2.5, 5.0, or 10 mM). After 30 and 120 min of incubation at 37 °C, samples were serially diluted and plated on BHI agar.
创建时间:
2021-11-25



