Expression data from NRK-52E cells treated with chlorothalonil for 6h, 24h and 72h.. Rattus norvegicus

Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) is a widely used broad spectrum fungicide. Despite its common use, chlorothalonil is classified by IARC as Group 2B, a possible human carcinogen, with formation of renal tubular adenomas and carcinomas in rats of both sexes and in male mice. In this study we have examined the effect of sub-cytotoxic exposure to chlorothalonil at 6h, 24h and 72h on the whole-genome expression profile in a rat proximal renal tubule cell line (NRK-52E). We used microarrays to detail the mechanism of toxicity and possibly carcinogenicity. Overall design: NRK-52E cells were cultured to confluence on 6-well plates. Cells were then exposed to chlorothalonil dissolved in DMSO (0.1%) at the IC10 concentration at 72h (1.1 μM CHL) or DMSO only as control. After 6h, 24h and 72h the medium was removed and RNA was extracted from the cells. Three studies were conducted at each time point.