Accumulation of Orf65+ viral particles at the nucleus of a KSHV-infected endothelial cell.

HUVEC were infected with KSHV and stained for Orf65+ viral capsids (red), actin cytoskeleton (green), and nuclei (blue) at 4 hpi. (A–B) An overview of a HUVEC infected by KSHV shown in a XY section (A, also see Video S1 for supporting information) and the corresponding YZ cross-section (B, also see Video S2 for supporting information). (C–D) Magnified 3D-projection image of the cell nucleus (C, also see a 3-D projection image in Video S3) and the corresponding cross-section of nucleus (D, also see Video S4 for supporting information).