Global and diGLY enriched proteomes of RanBPM depleted HeLa cells and RanBPM APMS

The human CTLH complex is a newly discovered multi-subunit E3 ligase. At present, only a few of its ubiquitination targets are known. Here, we use proteomic techniques in RanBPM-depleted HeLa cells to identify ubiquitination substrates of the CTLH complex. First, global proteomics determined proteins that were significantly increased in cells depleted of RanBPM. This analysis revealed potential degradation-induced ubiquitination substrates of the complex. Ubiquitination differences using diGLY-enriched proteomics in shRanBPM cells compared with the endogenous RanBPM interactome also revealed candidate ubiquitination targets.