Supplementary Material for: Body Composition following Necrotising Enterocolitis in Preterm Infants

<b><i>Background:</i></b> The optimal nutritional regimen for preterm infants, including those that develop necrotising enterocolitis (NEC), is unknown. <b><i>Objective:</i></b> The objective here was to evaluate body composition at term in infants following NEC, in comparison with healthy infants. The primary outcome measure was non-adipose tissue mass (non-ATM). <b><i>Methods:</i></b> We compared body composition assessed by magnetic resonance imaging at term in infants born &lt;31 weeks of gestational age that participated in NEON, a trial comparing incremental versus immediate delivery of parenteral amino acids on non-ATM, and SMOF versus intralipid on intrahepatocellular lipid content. There were no differences in the primary outcomes. We compared infants that received surgery for NEC (NEC-surgical), infants with medically managed NEC (NEC-medical), and infants without NEC (reference). <b><i>Results:</i></b> A total of 133 infants were included (8 NEC-surgical; 15 NEC-medical; 110 reference). In comparison with the reference group, infants in the NEC-surgical and NEC-medical groups were significantly lighter [adjusted mean difference (95% CI) NEC-surgical: –630 g (–1,010, –210), <i>p</i> = 0.003; NEC-medical: –440 g (–760, –110), <i>p</i> = 0.009] and the total adipose tissue volume (ATV) was significantly lower [NEC-surgical: –360 cm³ (–516, –204), <i>p</i> &lt; 0.001; NEC-medical: –127 cm³ (–251, –4); <i>p</i> = 0.043]. There were no significant differences in non-ATM [adjusted mean difference (95% CI) NEC-surgical: –46 g (–281, 189), <i>p</i> = 0.70; NEC-medical: –122 g (–308, 63), <i>p</i> = 0.20]. <b><i>Conclusion:</i></b> The lower weight at term in preterm infants following surgically and medically managed NEC, in comparison to preterm infants that did not develop the disease, was secondary to a reduction in ATV. This suggests that the nutritional regimen received was adequate to preserve non-ATM but not to support the normal third-trimester deposition of adipose tissue in preterm infants.