We report the application of single-molecule-based sequencing technology for transcription profile analysis of two S. cerevisiae strains. By combining the whole genome sequence of these strains,we sou
Overnight cultures of wild-type yeast were diluted in fresh medium with or without 10 mM sodium crotonate in the presence of 0.8 M sorbitol. After 3.5 hours cells were in early log-phase (1 × 10^7 cel