Nasal excretion of CA/04/09 in vaccinated ferrets.

Ferrets administered twice with 7.5 µl/dose HA of a-Brisbane/59/07, a-CAN01/04 or RgCA/04/09xPR8 were experimentally instillated i.n. with 105 TCID50 CA/04/09 virus challenge in a 1.0 ml volume. Aerosol transmission of the test virus was monitored by adding seronegative contacts in an isolator with 5 cm perforated separation barrier. Nasal wash specimens were obtained at 2, 4, 5, 6, 7, and 8 dpi. Virus titrations were done in embryonated chicken eggs (log10 EID50/ml) where the limit of virus detection was set to 0.7 log10 EID50/ml. Dash marks indicate no virus detection. *Standard deviation titers.