Source data for: Human monoclonal antibodies against Staphylococcus aureus surface antigens recognize in vitro biofilm and in vivo implant infections

Implant-associated Staphylococcus aureus infections are difficult to treat
because of biofilm formation. Bacteria in a biofilm are often insensitive
to antibiotics and host immunity. Monoclonal antibodies (mAbs) could
provide an alternative approach to improve the diagnosis and potential
 treatment of biofilm-related infections. Here we show that mAbs
targeting common surface components of S. aureus can recognize clinically
relevant biofilm types. The mAbs were also shown to bind a collection of
clinical isolates derived from different biofilm-associated infections
(endocarditis, prosthetic joint, catheter). We identify two groups of
antibodies: one group that uniquely binds S. aureus in biofilm state and
one that recognizes S. aureus in both biofilm and planktonic state.
Furthermore, we show that a mAb recognizing wall teichoic acid (WTA; clone
4497) specifically localizes to a subcutaneously implanted pre-colonized
catheter in mice. In conclusion, we demonstrate the capacity of several
human mAbs to detect S. aureus biofilms in vitro and in vivo.