Gene expression analysis in the Brainstem of Vglut2:Ndufs4cKO mice

Leigh Syndrome is characterized by symmetrical brain lesions and neuroinflammation in select nuclei, predominantly brainstem and/or basal ganglia. Accordingly, Ndufs4-deficient mice present overt lesions in brainstem, olfactory bulb, and basal ganglia. To define the contribution of Ndufs4 deficiency in excitatory neurons to the overall neuroinflammatory phenotype, we performed Gene expression analysis in the brainstem of mice with specific deletion of Ndufs4 in Vglut2-expressing neurons (Vglut2:Ndufs4cKO mice). This analysis allowed us to further define the inflammatory phenotype present in the brainstem of Vglut2:Ndufs4cKO mice. Total RNA extracted from the braistem of Vglut2:Ndufs4KO was compared to total RNA extracted from control (Vglut2:Ndufs4cCT) mice