Descriptive statistics of the five candidate reference genes, calculated BestKeeper Index and comparative analysis with RefFinder.

Data of expression for the five candidate reference genes (RGs), based on their crossing point (CP) values, were introduced in BestKeeper software. The result of descriptive analysis for every gene is showed in the left column. The Bestkeeper index was calculated for five genes (RPL13A, EEF1A1, CYC, GAPDH and 18S), for three genes (RPL13A, EEF1A1 and CYC) and for two genes (RPL13A and EEF1A1) (middle column). Data for the combination of 5 and 3 RGs were introduced in the RefFinder tool and the results are shown in the right column. Geometric Mean (GM), Arithmetic Mean (AM), Minimum (Min) and Maximum (Max) Crossing Point value (CP), Standard Deviation (SD) and Coefficient of Variation (CV) * indicates when the value is out of range.