Supporting data for "Antiviral Activities of Live Attenuated Influenza Virus-vectored Vaccine Against SARS-CoV-2 Infection in Lung Epithelial Cells"

This is the complete dataset for the thesis "Antiviral activities of live attenuated influenza virus-vectored vaccine against SARS-CoV-2 infection in lung epithelial cells".Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and Influenza A virus (IAV) are two of the major respiratory disease-inducing viruses that have caused global pandemic. The co-circulation of the two viruses is of concern for posing potential threat to public health. However, the characteristic of IAV triggering a robust and early innate immune response makes IAV-vectored virus particle a potential tool for therapeutic strategy for management of SARS-CoV-2 infection. This research study aimed to assess the efficacy of using live attenuated influenza virus vector-based COVID19 vaccine as antiviral intervention against SARS-CoV-2 infection, by the use of cell-based in vitro models involving human or mouse lung epithelial cells as well as immune cells.All experiments involving live SARS-CoV-2 viruses were conducted following approved standard operative procedures in biosafety level 3 facility in the Department of Microbiology of The University of Hong Kong.Detailed methodology can be found in the "Chapter 2. Materials and Methods" of the thesis.All data are included in the dataset main folder. The file directory and description for the dataset can be found in the README.txt file.

本数据集完整收录了题为《活疫苗减毒流感病毒载体对肺部上皮细胞中SARS-CoV-2感染抗病毒活性》的论文数据。严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）与甲型流感病毒（IAV）均为导致全球性呼吸道疾病大流行的两大主要病毒。两种病毒的共循环状况令人担忧，可能对公共卫生构成潜在威胁。然而，IAV引发强大且早期的先天免疫反应的特性，使得IAV病毒载体颗粒成为管理SARS-CoV-2感染的治疗策略中的潜在工具。本研究旨在评估使用活疫苗减毒流感病毒载体为基础的COVID19疫苗作为抗病毒干预措施对SARS-CoV-2感染的有效性，通过涉及人类或小鼠肺部上皮细胞以及免疫细胞的细胞培养体外模型进行评估。所有涉及活SARS-CoV-2病毒实验均遵循香港大学微生物学系生物安全等级3设施中批准的标准操作程序进行。详细的实验方法可查阅论文的“第二章 材料与方法”部分。所有数据均包含在数据集主文件夹中。数据集的文件目录和描述可查阅README.txt文件。