Supplementary Table S4 and S5 for manuscript "Evolutionary rates of bumblebee genomes are faster at lower elevations"

Concatenated orthologous sequences of 14 bumblebees
species (Bombus spp.).

Bumblebees (12
species) were sequenced with both directions of 150 bp reads representing
approximately 100×coverage of the genome. The clean reads were used for de novo
assembly with IDBA-UD. Previously published genomes of B. terrestris (RefSeq
assembly accession: GCF_000214255.1) and B. impatiens (RefSeq assembly
accession: GCF_000188095.2) were also used.

The coding
sequence of the longest exon of each nuclear gene (CDS-L) was extracted. The
orthologous CDS-L sequences among the 14 species were identified and aligned.
All the nuclear sequences (CDS-L) were concatenated into the NUC file (Supplementary_Table_S4(NUC).txt,
in fasta format). The 13 coding genes in the mitochondrial genome were
extracted and aligned manually. All the mitochondrial sequences were concatenated
into the MIT file with fasta format (Supplementary_Table_S5(MIT).txt, in fasta
format).