Interaction of muscle and liver circadian clocks on the transcriptome. Interaction of muscle and liver circadian clocks on the transcriptome

In this study, we used RNA sequencing to interrogate how muscle and liver autonomous clocks influence the circadian transcriptome across these two organs. Overall design: WT and liver+muscle Bmal1 reconstituted mice ( see Koronowski et al Cell 2019 and Greco et al Science Advances 2021 for general model description) were used to collect tissues at 6 time points over the diurnal cycle - 12 hr light: 12 hr dark conditions and ad libitum feeding of normal chow; tissues harvested at ZT0, 4, 8, 12, 16, 20