Summary of cross-reactive arenavirus peptides.

aPosition of epitope and cross-reactive peptide within prototypic strains of arenavirus GPC and NP.
bFull amino acid sequence is shown for epitope/immunizing peptide. Boldface letters indicate amino acid differences between epitope and cross-reactive arenavirus peptide.
cCD8+ T cells from peptide-immunized HLA-A*0201 and HLA-A*1101 transgenic mice were tested for their ability to produce IFN-γ in response to HLA-restricted target cells that had been pulsed with peptide. Percent of epitope response is calculated according to the formula: [(net spots/106 CD8+ T cells against cross-reactive peptide)/(net spots/106 CD8+ T cells against epitope)×100]. Data are representative of at least two independent experiments.
dPeptides were tested for binding to their respective HLA allele. Bold font highlights biologically relevant binders (IC50≤500 nM). −, Peptides with binding affinity >5000 nM. Binding affinities for LCMV GPC447–455, LASV GPC441–449, and WWAV GPC428–436 peptides are referenced from [10] and J. Botten and M. J. Buchmeier, unpublished work.