Zebrafish establish female germ cell identity by advancing cell proliferation and meiosis

In this study, we utilize the RNA-seq of germ cell to uncover the early germ cell development. To obtain fish with defined sex, we crossed domesticated zebrafish with the Nadia strain that has a female-dominant W segment. These fish were placed on a ziwi:GFP background to facilitate sorting of fluorescent germ cells for transcriptomic analysis. We analyzed the transcriptomes of germ cells at 10 to 14 days postfertilization (dpf), when sex dimorphic changes started to appear. Germ cells mRNA of 10-,12- and 14-day post fertilization in W-hybrid (ZW*Tupfel long-fin) and Z-hybrid (ZZ*Tupfel long-fin)