RNA sequencing of human peripheral blood cDC1 treated with PGE2

RNA sequencing was used to characterize PGE2-mediated changes in the gene expression profile of human conventional type 1 dendritic cells (cDC1) purified from PBMCs of healthy donors. Our analysis shows that treatment of cDC1 with PGE2 induces transcriptional changes in resting cDC1. cDC1 activated with a TLR3 ligand after PGE2 pre-treatment show alterations in the expression of activation induced genes. Overall design: cDC1 (CD370+CD141+CD3- cells) were sort purified from PBMCs of four healthy donors (A,B,C,D). cDC1 were treated or not with PGE2 (1µg/ml) for 24h. In some groups, the TLR3 ligand poly I:C was added to the culture for the last 4h of the experiment to induce cDC1 activation.