Gene expression profiling in the puf4∆ (midlog), and gcn4∆ (midlog and oxidative stress) mutants of Cryptococcus neoformans.. Gene expression profiling in the puf4∆ (midlog), and gcn4∆ (midlog and oxidative stress) mutants of Cryptococcus neoformans.

This study aimed to investigate transcriptomic changes in Cryptococcus neoformans mutants. We report transcriptome wide changes in the puf4∆ under basal growth conditions (30˚C, midlog, YPD). We report transcriptome remodeling following hydrogen peroxide (H2O2) treatment in wildtype (H99) and gcn4∆ cells. Overall design: Cells were grown to midlog growth stage at 30˚C, then indicated samples were treated with 1 mM H2O2 for 1 hour. Cells were pelleted and total RNA were extracted, DNase treated and sequenced using Illumina HiSeq platform.