Melioidosis DS assay results for the re-evaluation of the previously misclassified sera.

Melioidosis DS assay signal intensities for the four spotted antigens tested with Hcp1 singleplex LFA false-negative melioidosis sera and false-positive controls. Signal intensities were obtained by comparison of the respective band intensities to the gold reference card (Senova, Germany). Furthermore, a binary representation (positive—“1”/negative—“0”) is shown for each band besides the overall number of positive bands per assay. Finally, the Melioidosis DS assay result is shown for two conditions: (1) at least one positive band, (2) at least two positive bands. (XLSX)