RNA-seq raw data (FASTQ) from Huvec cells over expressing or not miR-18b-3p
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RNAseq of total RNA from n=4 separate replicates of both mimic CTRL and mimic 18b-3p was performed on and Illumina HiSeq 2500 platform according to the following parameters: Hiseq2500 4 plex run, 2 × 100 bp reads, about 60 M reads/sample (for mRNAs/lincRNAs) after preparing the libraries with the Truseq stranded with RiboZero Illumina kit. RNA-seq data were analyzed with “rnaseq” version 3.9 pipeline included in the nf-core platform (https://nf-co.re/rnaseq/3.9) using default parameters. The alignment was performed using Star to map the FastQ reads to the reference genome and perform downstream BAM-level quantification with Salmon Normalized TPM values were used to determinate gene modulation among different condition.
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Zenodo创建时间:
2025-10-24



