Effect on gene expression of addition of N-acetylmannosamine to galactose grown Streptococcus pneumoniae.

RNA-seq analysis of N-acetylmannosamine upon growth and gene expression in pneumococci grown with galactose as sole sugar. Overall design: Pneumocccal strain D39 was grown to mid exponetial phase in chemically defined media (CDM) containing 55 mM galactose (as sole sugar). N-acetylmannosamine was added at 3.5 mM and samples were taken for RNA analysis at 0, 15 and 30 minutes post addition. Additionally separate D39 cultures were grown with 3.5 mM N-acetyl-mannosamine as the sole sugar and RNA extracted at mid-exponential.