Advancing Lung Tissue Engineering Through the Discovery of Factors that Control Alveolar Epithelial Cell Fate (snRNA-seq from recellularized scaffolds)

The objective of this study was to evaluate primary, human AT2 cells following seeding into decellularized lung scaffolds. Overall design: Alveolar type 2 cells were isolated from human lung tissue, expanded in large-scale, and seeded onto decellularized porcine lung scaffolds. Cell culture media was used to promote AT1 differentiation, and samples were taken down over a time-course study. SnRNA-seq analysis was performed on the recellularized lung tissue.