Data for: A pilot study of inflammatory mediators in brain extracellular fluid in paediatric tuberculous meningitis (TBM)

This is the data linked to: A pilot study of inflammatory mediators in brain extracellular fluid in paediatric TBM (2021), PlosOneTuberculous meningitis (TBM) is the most fatal form of tuberculosis
and frequently occurs in children. The inflammatory process initiates
secondary brain injury processes that lead to death and disability. Much
remains unknown about this cerebral inflammatory process, largely
because of the difficulty in studying the brain. To date, studies have
typically examined samples from sites distal to the site of disease,
such as spinal cerebrospinal fluid (CSF) and blood. In this pilot study,
we examined the feasibility of using direct brain microdialysis (MD) to
detect inflammatory mediators in brain extracellular fluid (ECF) in
TBM. MD was used to help guide neurocritical care in 7 comatose children
with TBM by monitoring brain chemistry for up to 4 days. Remnant ECF
fluid was stored for offline analysis. Samples of ventricular CSF,
lumbar CSF and blood were collected at clinically indicated procedures
for comparison. Inflammatory mediators were quantified using multiplex
technology. All inflammatory markers, with the exception of interleukin
(IL)-10 and IL-12p40, were detected in the ECF. Cytokine concentrations
were generally lower in ECF than ventricular CSF in time-linked
specimens. Individual cases showed ECF cytokine increases coinciding
with marked increases in ECF glycerol or decreases in ECF glucose.
Cytokine levels and glycerol were generally higher in patients with more
severe disease. This is the first report of inflammatory marker
analysis from samples derived directly from the brain and in high
temporal resolution, demonstrating feasibility of cerebral MD to explore
disease progression and possibly therapy response in TBM.Reason for stand-alone publication:This raw data which was analysed to put together the paper. It has been made public inline with PlosOne's publication requirements.

本数据集关联的研究为《2021年儿童结核性脑膜炎（TBM）脑外周液炎症介质的试点研究》，发表于《PlosOne》。结核性脑膜炎作为结核病中最致命的形式，常发生于儿童。炎症过程引发继发性脑损伤，导致死亡和残疾。由于研究大脑的困难，关于这一脑部炎症过程，尚有许多未知之处。迄今为止，研究通常考察疾病原位远端的样本，如脊髓脑脊液（CSF）和血液。在本项试点研究中，我们探讨了利用直接脑微透析（MD）技术在TBM患者的脑外周液（ECF）中检测炎症介质的方法。MD技术被用于指导7名昏迷TBM儿童的神经重症监护，通过监测大脑化学物质至4天。剩余的ECF液体被储存以供离线分析。在临床指征的程序中收集了脑室CSF、腰段CSF和血液样本以进行对比。使用多重技术对炎症介质进行定量分析。除白细胞介素（IL）-10和IL-12p40外，所有炎症标记物均检测到ECF中。在时间相关的标本中，与脑室CSF相比，ECF中的细胞因子浓度通常较低。个别病例显示，与ECF甘油显著增加或葡萄糖减少同时，ECF细胞因子浓度上升。在病情更严重的患者中，细胞因子水平和甘油通常较高。这是首次报道从脑部直接获取并具有高时间分辨率的炎症标记物分析，展示了脑部MD在探索TBM疾病进展和可能的治疗反应中的可行性。独立发表的原因：这些原始数据是撰写论文所分析的数据。根据PlosOne的出版要求，已将其公开。