Analysis of the Soluble ATP-Binding Proteome of Plant Mitochondria Identifies New Proteins and Nucleotide Triphosphate Interactions within the Matrix

The interactions of ATP inside plant mitochondria
were investigated by identifying the soluble nucleotide
binding proteome captured using immobilized ATP. Selected proteins were separated by 1D SDS-PAGE and 2D
IEF-SDS-PAGE and identified by ESI−Q-TOF MS/MS. A
range of highly enriched proteins were identified from the
mitochondrial proteome, including 14-3-3 proteins and
RNA binding proteins, as well as proteins known to
contain nucleotide binding domains and/or to be inhibited
or stimulated by ATP.
Keywords: plant mitochondria • Arabidopsis • ATP affinity • ATP
synthesis • respiration