Transcriptomic Analysis Reveals the Responses of Dendritic Cells to Vdbp

Our current study overexpressed Vdbp stably in DC to explore the function of this gene. CKK-8 results revealed Vdbp successfully inhibited viability of DC. Besides, we found overexpressing this gene greatly promoted apoptosis and obviously altered cell cycle distribution in DC. Moreover, RNA sequencing (RNA-seq) was carried out and numerous differently expressed genes (DEGs) were obtained. The results indicated that Vdbp globally mediated expression of mRNAs, which might translate into its potential functions. Finally, bio-informatics analysis illustrated that most DEGs were more uniformly enriched in immunity-related pathways. We believe that Vdbp regulates the function of DC by altering its cellular immunity. Overall design: Examination the overexpression of Vdbp in DCs.