Transcriptional profiling of mouse plasma cells (population RNA-Seq)

CD138-high plasma cell subsets were analyzed by RNA-sequencing to identify gene expression differences between long- and short-lived plasma cells. Five different CD138-high subsets were analyzed based upon differential B220 expression and uptake of the fluorescent glucose analog 2NBDG: splenic B220+2NBDG-, B220+2NBDG+, B220-2NBDG-, B220-2NBDG+, and bone marrow B220-2NBDG+ cells. Approximately 30,000 cells were analyzed of each subset. Three biological replicates of each subset were included. Samples were double sorted from 8-12 week old C57Bl6/N male mice.