Single-cell profiling reveals immune-based mechanisms underlying tumor radiosensitization by a novel Mn porphyrin clinical candidate, MnTnBuOE-2-PyP5+ (BMX-001)

Manganese porphyrins reportedly exhibit synergic effects when combined with irradiation. However, an in-depth understanding of intratumoral heterogeneity and immune pathways, as affected by Mn porphyrins, remains limited. Here, we explored the mechanisms underlying immunomodulation of a clinical candidate, MnTnBuOE-2-PyP5+ (BMX-001, MnBuOE), using single-cell analysis in murine carcinoma model. Mice bearing 4T1 tumors were divided into 4 groups: control, MnBuOE, radiotherapy (RT), combined MnBuOE, and radiotherapy (MnBuOE/RT). In epithelial cells, epithelial-mesenchymal transition, TNF-α signaling via NF-кB, angiogenesis, and hypoxia-related genes were significantly downregulated in the MnBuOE/RT compared to the RT. All subtypes of cancer-associated fibroblasts (CAFs) were reduced in MnBuOE and MnBuOE/RT. Inhibitory receptor-ligand interactions, in which epithelial cells and CAFs interacted with CD8+ T cells, were significantly lower in the MnBuOE/RT than in the RT. Trajectory analysis ..., , , # Single-cell profiling reveals immune-based mechanisms underlying tumor radiosensitization by a novel Mn porphyrin clinical candidate, MnTnBuOE-2-PyP5+ (BMX-001)

[https://doi.org/10.5061/dryad.b2rbnzspd](https://doi.org/10.5061/dryad.b2rbnzspd)

The dataset comprises filtered matrices generated by CellRanger **(barcodes.tsv, features.tsv, matrix.mtx)**, along with single-cell annotated metadata **(Major_cell_annotations.txt)**, intended for publication in Antioxidants 2024

## Descriptions

### **Single-cell sequencing data**

\-  **barcodes.tsv**: the barcodes (unique cell-id) saved as TSV format that corresponds to 10x formats

\-  **features.tsv**: the features/genes saved as TSV format that corresponds to 10x formats

\-  **matrix.mtx**: the matrix files are stored in MTX  corresponding to 10x formats.

\-  **Major_cell_annotations.txt**: a meta-data file is included that contains the following columns.

\- **barcodes**: Contains unique cell barcodes. The prefix \"Bx-Px\" is concat...