Effects of different efficient planting modes on crop rhizosphere soil microorganisms

Soil samples were conducted in Yanzi Village, Lingxi Town, Cili County, Zhangjiajie City, Hunan Province, and samples were taken in July and November 2024, respectively. According to the five-point sampling method, 200g of 0-20cm rhizosphere soil was taken from each plot, and after removing litter, gravel and other debris, the samples were placed in 50mL centrifuge tubes with lids, stored in an incubator with dry ice packs, and then sent to Jiangsu Nantong Sanshu Biotechnology Co., Ltd. for metagenomic sequencing in time