Characteristics of organisms in the simulated metagenome.

For each organism in the mixture, we give genome and organism characteristics alongside statistics for various stages of sample preparation. The cell density was determined by flow cytometry. The stock cultures were mixed such that each organism contributed an equal number of cells to the mixture. DNA from an aliquot of each culture was extracted using the EnzBB method and quantified. That quantity is used to calculate an expected representation of the organisms as a percentage of sequence reads, given in the column “% expected in metagenomic reads.”