Example live-cell imaging datasets for "A modular live-cell biosensor for extracellular protease activity"

A small example subset of the live-cell fluorescence microscopy dataset accompanying Ramm, Weatherly & Toettcher (2026), A modular live-cell biosensor for extracellular protease activity, bioRxiv 10.64898/2026.06.19.733436. This is a curated, small subset of the imaging data, not the complete dataset, selected so that the published analysis pipeline can be tested. Outputs computed from this dataset will not fully match the published figures. The full raw dataset is available from the corresponding authors on request. The data show eNRGies (engineered neuregulin reporters as generalized indicators of extracellular shedding), modular genetically encoded biosensors that convert extracellular proteolytic cleavage into nuclear translocation of a fluorescent reporter domain. Included are time-lapse experiments with soluble proteases (TEV protease) and endogenous sheddase activity (ADAM17), imaged together with either segmentation markers or a second biosensor for ERK activity (ERK-KTR). Contents: multi-channel tiff time-lapse stacks of HEK293T or MCF10A cell lines with accompanying segmentation/tracking outputs, organised under example_images: - cellpose_segmentation: raw stack for the Cellpose 3 segmentation example- horizontal_barplot: biosensor variant comparison (TEVp) - main_plots: biosensor time courses, PBS vs. TEV protease dose series- tracking_ERK-KTR_eNRGies: concurrent imaging of eNRGies and ERK-KTR, single-cell tracking- tracking_mitosis: data for single-cell tracking of dividing HEK293T cells Usage: analysis code (Jupyter notebooks and Python functions) is available at github.com/BeaRamm/Ramm2026. Download example_images.zip and extract it at the repository root; the archive already contains the example_images/ folder, so the notebooks' relative paths work without further changes.