Data from: Development and characterization of 15 polymorphic di-nucleotide microsatellite markers for tule elk using HiSeq3000

The tule elk (Cervus elaphus nannodes) experienced a severe bottleneck in
the 1800s, resulting in low genetic diversity. There is a need for
high-resolution genetic assays that can be used to differentiate
individual elk, including close relatives, with high confidence. An
efficient assay requires multiple markers both polymorphic and that can be
amplified in concert with other markers in multiplex reactions. To develop
such markers, we employed 150-bp paired-end whole genome shotgun
sequencing on an Illumina HiSeq3000 platform to discover dinucleotide
microsatellite markers. After preliminary screening of these markers, we
selected and screened 15 candidate loci and 5 existing tetra nucleotide
markers in 56 tule elk. We combined these markers in 2 multiplex reactions
and report primer concentrations and PCR conditions enabling their
efficient amplification.