Effects of marking, chilling and mechanical separation on the blood feeding success, fecundity and fertility of wMel-infected Aedes aegypti.

In field trial II (Nausori 2019), mosquitoes were sampled from the emergence cages (rearing control), after cold-immobilization at 4°C for 30 minutes (immobilization control) and at the end of UAV flights (aerial). Collected mosquitoes were sorted into cages of 50 females and 15 males. Females were allowed to blood feed and the blood feeding success was determined. Blood fed females were kept and allowed to oviposit and fecundity is shown as eggs per female. Eggs were hatched and larvae were counted 48 hours post hatching to determine fertility. Statistical analysis was performed using ordinary one-way ANOVA. NS = Not statistically significant and ND = Not done.