Reduced number of infiltrating activated T lymphocytes by transplantation of rfNSCs-IDO.

The EAE model exhibited the abnormal infiltration of lymphocytes into the brain. (A) Immunofluorescent staining was performed against CD3+ T lymphocytes, CD25+ activated T cells, and FoxP3+ regulatory T cells in cerebral cortex. Scale bars, 100 μm. (B) The number of lymphocytes present in the cerebral cortex was quantified. The number of CD3+ T cells was not different among experimental groups whereas the number of CD25+ activated T cells was significantly reduced in both the rfNSCs-Cont and the rfNSCs-IDO groups compared to the HBSS group. The number of FoxP3+ regulatory T cell was greatly increased in the rfNSCs-Cont and the rfNSCs-IDO groups, and the rfNSCs-IDO group showed a significantly increased level compared to the rfNSCs-Cont group (n = 4, * p p