Non-alcoholic steatohepatitis disrupts diurnal liver transcriptome rhythms in mice

Circadian profiling improves target gene detection and pathway characterization in mouse models of NASH. Fatty livers show 3-hour advances in metabolic and clock gene transcript rhythms. Circadian profiling further reveals temporal changes in lipid, carbohydrate, and cholesterol metabolic transcripts. Overall design: Mice were fed with choline free methionine low high fat diet (cdHFD) or normal chow during 2 weeks. Mice were then sacrificed every 6 h and liver was collected. Rnaseq was then performed and data analyzed using different methods.