Tissue- and site-specific DNA recombination in transgenic mice.
收藏PubMed Central1992-08-01 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC49604/
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资源简介：
We have developed a method of specifically modifying the mammalian genome in vivo. This procedure comprises heritable tissue-specific and site-specific DNA recombination as a function of recombinase expression in transgenic mice. Transgenes encoding the bacteriophage P1 Cre recombinase and the loxP-flanked beta-galactosidase gene were used to generate transgenic mice. Genomic DNA from doubly transgenic mice exhibited tissue-specific DNA recombination as a result of Cre expression. Further characterization revealed that this process was highly efficient at distinct chromosomal integration sites. These studies also imply that Cre-mediated recombination provides a heritable marker for mitoses following the loss of Cre expression. This transgene-recombination system permits unique approaches to in vivo studies of gene function within experimentally defined spatial and temporal boundaries. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1992-08-01
