Cortical miR-709 links glutamatergic signaling to NREM sleep EEG slow waves in an activity-dependent manner [RNA-seq]

Micro-RNAs (miRNAs) are key, post-transcriptional regulators of gene expression that have been implicated in a plethora of biological processes. Their role in sleep, however, has received little attention. In the current experiment, we investigated the effects of in vitro miR-709 inhibition on the transcriptome of primary cortical neurons. Overall design: Eighteen primary cortical neuronal cultures from C57BL6/J mice were distributed in 3 groups, each one following a different treatment, namely miR-709 inhibition (i-miR-709, n=6), scrambled control (scr, n=6) or ddH2O control (ctrl, n=6), and their transcriptome was subsequently assessed by RNA-sequencing.