TABLE 1 in A new genus in the tribe Bothriocerini (Hemiptera: Auchenorrhyncha: Fulgoroidea: Cixiidae) from the cloud forests of Costa Rica, a key to genus, and an updated molecular phylogeny

TABLE 1. Primers used to amplify corresponding gene regions used to assess the placement of novel taxon and PCR parameters for each locus. LocusPrimerDirectionSequence (5’ 6 3’)AnnealingExtensionReferenceCOI (5’)COI_D1_FForwardGGAACWATAAGAAGWATAATYATYCG40˚C1 min. 30 sec.Humphries et al. 2021C1-J-2195RCReverseACTTCTGGATGACCAAAAAATCAACOI (3’)C1-J-2195ForwardTTGATTTTTTGGTCATCCAGAAGT40˚C1 min. 30 sec.Simon et al. 1994TL2-N-3014ReverseTCCAATGCACTAATCTGCCATATTA18S18SFForwardGGATAACTGTGGTAATTCTAG50˚C2 min.Bahder et al. 201918SRReverseGTCCGAAGACCTCACTAAAH3H3F2ForwardGKAARTCSACCGGHGGHAARGC55˚C30 sec.Bahder et al. 2023 Echavarria et al. 2021H3RReverseGTKACHCKCTTRGCGTGRAT28S (D8)LaltForwardCCTCGGACCTTGAAAATCC55 ˚C1 min. 30 sec.Cryan et al. 2000GaltReverseTGTCTCCTTACAGTGCCAGA28S (D9–D10)VForwardGTAGCCAAATGCCTCGTCA55 ˚C1 min. 30 sec.Cryan et al. 2000XReverseCACAATGATAGGAAGAGCC