High-throughput Y2H sequencing

NIAID Data Ecosystem2026-03-14 收录

蛋白质相互作用

高通量酵母双杂交

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA914778 数据链接链接失效反馈

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资源简介：

we developed a high-throughput method by combining yeast two-hybrid (Y2H) with next-generation sequencing (NGS) technology to screen the interactions between receptors and cargoes

创建时间：

2022-12-21

Activated FGFR3:p-FRS2:p-PPTN11 binds GRB2:GAB1:PI3KR1

蛋白质相互作用

生物信息学

This event has been computationally inferred from an event that has been demonstrated in another species. The inference is based on the homology mapping from PANTHER. Briefly, reactions for which al

reactome.org90

Data_Sheet_1_Effect of Mutations in GvpJ and GvpM on Gas Vesicle Formation of Halobacterium salinarum.pdf

分子生物学

蛋白质相互作用

The two haloarchaeal proteins, GvpM and GvpJ, are homologous to GvpA, the major gas vesicle structural protein. All three are hydrophobic and essential for gas vesicle formation. The effect of mutatio

NIAID Data Ecosystem40

Proteins co-precipitated with the EIF4AI wild-type.

蛋白质相互作用

亲和纯化质谱

Only proteins represented by a minimum of two peptides in at least two of the replicates from the bait and with an enrichment ratio in comparison with the control equal to or greater than 1.5 were con

NIAID Data Ecosystem00

Validation by comparing NBEL algorithm with naïve Bayes via two human datasets.

蛋白质相互作用

算法验证

In table 3, indicates the total number of protein pairs in a database that are overlapped with our collected 79,441 human PPIs; indicates the number of protein pairs in that are predicted by NBEL;

NIAID Data Ecosystem80

Table 5_Successful prediction of LC8 binding to intrinsically disordered proteins sheds light on AlphaFold’s black box.xlsx

蛋白质相互作用

结构生物信息学

IntroductionLC8 is a hub protein involved in many processes from tumor suppression and cell cycle regulation to neurotransmission and viral infection. Despite recent progress, prediction of binding si

NIAID Data Ecosystem00

High-throughput Y2H sequencing

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