Left-right defects in sec10MO embryos.

Left-right defects assayed at the level of asymmetric gene expression
and organ placement as described [31]. Embryos
were scored by in situ hybridization for asymmetric
gene expression of spaw (in the lateral plate
mesoderm), lefty2 (in the heart), and
lefty1 (in the diencephalon) by in
situ hybridization at approximately 19 somite stage
(19ss). Embryos were scored for the laterality of organ placement
using myl7 (in the heart), foxa3
(in the liver), and insulin (in the pancreas) at 2
dpf. Situs
solitus = right-looped heart,
left-sided liver, right-sided pancreas. Situs
inversus = left-looped heart,
right-sided liver, left-sided pancreas.
Heterotaxia = all other organ conformations.
P values reported are from Fisher's exact
test, comparing the given row against uninjected embryos. We also
employed the same test to compare the effects of the various
treatments. All treatments differed from one another in their
effects on both gene expression and organ placement
(p = 0.003–2.08x10-7)
except for the effects of 4ng pkd2MO and 8+8ng sec10MO on organ
placement, which were statistically indistinguishable
(p = 0.19).