Comparative proteomic and phosphorylated proteomic analyses revealed differences in sperm motility in Mediterranean buffalo

In this study, Mediterranean buffalo semen was used as experimental material, and quantitative proteomics and phosphorylation modification techniques were used to analyze the proteins and phosphorylation sites of Mediterranean buffalo sperm with different motility, so as to further study the protein functions affecting sperm motility, providing a theoretical basis for understanding the regulatory network related to sperm motility and improving the sperm quality and fertilization ability of Mediterranean buffalo.