RNA virome of sylvatic mosquitoes from northeast Brazil. RNA virome of sylvatic mosquitoes from northeast Brazil

Here, we sequenced the RNA virome of 10 sylvatic mosquitos species from Atlantic forest remains at a sylvatic-urban interface in Northeast Brazil employing a metatranscriptomic approach. The RNA samples were extracted from pooled mosquito samples from the same species using Trizol reagent. RNA samples were quantified and quality checked through Qubit RNA HS kit and Bioanalyzer respectively. The RNA samples were processed for the ribosomal RNA depletion with the RiboMinus™ Eukaryote System v2 kit following manufacturer's instructions and the sequencing library was prepared using the TruSeq Stranded Total RNA library kit and sequenced on a NextSeq 500 Illumina platform using a paired-end approach of 75 bp.