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In the administration groups, (-)-α-bis was preadministered to HSF cells for 12h and then HSF cells were treated with 40 mg/mL D-Gal to induce senescence, senescence β-galactosidase staining was utilized, TNF-α, IL-6, IL-8, IL-1β, CCL-2, CCL-5 and MMP-9 in SASP were detected by RT-qPCR technology, and cell cycle-related proteins P16 and P21 were also measured by western blotting in D-Gal-induced HSF cells. Meanwhile, aged BALB/c mice were applied topically with 0.5% and 2%(-)-α-bis gel for 30 days continuously to evaluate anti-aging parameters on skin such as surface examination, the TEWL, skin barrier index of dorsal skin. Then, HE staining, Masson staining and IHC were applied to measure epidermal thickness, collagen fibers content in the dermis and content of dermal collagen Ⅰ, respectively. Moreover, RT-qPCR method was used to detect the mRNA expression levels of elastin, collagen Ⅲ, MMP-2 and MMP-3. Last, SOD , MDA and HYP contents of the back skin tissues of mice were also detected in this research.