<p>Enzymatic activity of chimeric variants obtained by the fusion of KP32gp38 and K11gp0043 domains. A blue colour indicates a KP32 origin, whereas grey indicates a K11 origin. LIN = linker between enzymatic domain and CBM; CBM = carbohydrate-binding module; LEC = lectin-like domain. NO = protein expression was observed on SDS-PAGE, but no halo was observed; nd = protein expression was not detected on SDS-PAGE, and a halo was not formed on tested lawns (K31, K21, KL163). The colour scheme and abbreviations correspond to the models presented in Fig 2. The green triangle indicates where a two-amino acid position marker was added. * inconsistent results for KL163. The 3D structures predicted using AlphaFold 3 [27] can be found in Fig D</p>

Enzymatic activity of chimeric variants obtained by the fusion of KP32gp38 and K11gp0043 domains. A blue colour indicates a KP32 origin, whereas grey indicates a K11 origin. LIN = linker between enzymatic domain and CBM; CBM = carbohydrate-binding module; LEC = lectin-like domain. NO = protein expression was observed on SDS-PAGE, but no halo was observed; nd = protein expression was not detected on SDS-PAGE, and a halo was not formed on tested lawns (K31, K21, KL163). The colour scheme and abbreviations correspond to the models presented in Fig 2. The green triangle indicates where a two-amino acid position marker was added. * inconsistent results for KL163. The 3D structures predicted using AlphaFold 3 [27] can be found in Fig D