Algorithms and information, fundamental to technological and biological organization, are also an essential aspect of many elementary physical phenomena, such as molecular self-assembly. Here we repor
Herein we propose a fully in vitro iterative method based on Golden Gate Aseembly to faithfully construct a long synthetic DNA molecule starting from commercially available double-stranded DNA. The sy
A problem rarely tackled by current DNA assembly methods is the issue of cloning additional parts into an already assembled construct. Costly PCR workflows are often hindered by repeated sequences, an
